Module 2/5 · Weeks 4–6 · 27 h

Multispectral data

UAT 365 Unmanned Aircraft Systems Technology for Smart Agriculture and Remote Sensing

About 90 minDraft, awaiting reviewLast updated 27 September 2026

Lesson

By the end of this module you will be able to

  1. Distinguish pixel values (DN) from reflectance and explain why calibration is needed
  2. Explain the roles of the calibrated reflectance panel (CRP) and the downwelling light sensor (DLS)
  3. Calibrate images with the empirical line method from two reference panels
  4. Explain how changing light during flight and band alignment affect vegetation indices

Prerequisites: UAT 365 module 1

Why this matters

The values in a camera image are digital numbers (DN), which depend on the sunlight at the time, the camera settings and the sensitivity of each band. The same rice leaf photographed in bright sun and under cloud gives very different DNs. Compute a vegetation index directly from DN, compare two weeks, and you may conclude the crop got worse when it was only cloudier that day. Calibration converts DN to reflectance, a property of the surface itself, so it can be compared across days and cameras.

Pixel values and reflectance

Reflectance is the fraction of incoming light a surface reflects, from 0 to 1. It is like measuring how white a sheet of paper is, which should not change whether the lights are bright or dim. DN is like the brightness you see in a photo, which does change with the light.

The MicaSense RedEdge-P comes with two items for this:

  • Calibrated reflectance panel (CRP) with known reflectance in each band, photographed before and after each flight
  • Downwelling light sensor (DLS2) on top of the drone, measuring incoming sunlight in each band throughout the flight, used to correct for changing light
Five steps from left to right: photograph the reference panel, fly with the DLS light sensor, align bands, convert DN to reflectance, and compute indices
Figure 1. Multispectral calibration workflow

The empirical line method

The empirical line method (Smith and Milton, 1999) uses at least two targets of known reflectance, fits a straight line between DN and reflectance, and applies that line to every pixel in the same band. The USGS report (2023) highlights this two-panel method for drone work. It must be done band by band, because each band has a different sensitivity.

A graph with pixel value DN on the horizontal axis and reflectance on the vertical axis. Two gold points are the dark 5 percent panel and the bright 50 percent panel. A blue straight line passes through them. Two green points on the line are crop pixels converted to reflectance
Figure 2. Empirical line from two reference panels (red band)

Example 1. Calibrating two bands and computing NDVI

The assumed panels have 5% and 50% reflectance in both bands. Panel and leaf DNs are assumed values.

PANELS = (0.05, 0.50)                          # reflectance of the dark and bright panels
panel_dn = {"red": (3200, 26000), "nir": (2500, 21000)}
crop_dn = {"red": 5000, "nir": 20000}          # DN of the rice leaf in each band

def calibrate(dn, dn_pair, rho_pair=PANELS):
    gain = (dn_pair[1] - dn_pair[0]) / (rho_pair[1] - rho_pair[0])
    offset = dn_pair[0] - gain * rho_pair[0]
    return (dn - offset) / gain

rho = {band: calibrate(crop_dn[band], panel_dn[band]) for band in crop_dn}
ndvi_rho = (rho["nir"] - rho["red"]) / (rho["nir"] + rho["red"])
ndvi_dn = (crop_dn["nir"] - crop_dn["red"]) / (crop_dn["nir"] + crop_dn["red"])
print(f"reflectance red {rho['red']:.3f}, NIR {rho['nir']:.3f}")
print(f"NDVI from reflectance {ndvi_rho:.3f} vs from raw DN {ndvi_dn:.3f}")
reflectance red 0.086, NIR 0.476
NDVI from reflectance 0.695 vs from raw DN 0.600

NDVI from DN is clearly lower than the true value because the two bands differ in sensitivity and offset. Numbers from raw DN cannot be compared with other work or with thresholds in the literature.

Light changing during flight

If the panel is photographed in bright sun but cloud covers part of the field during the flight, those pixels get reflectance that is too low. The DLS measures light at each image, so it can correct by the light ratio. Cloud reduces light by different amounts in each band, so vegetation indices are affected too, not just reflectance.

Example 2. The effect of cloud without DLS correction

Assume thin cloud reduces light to 70% in the red band and 80% in NIR compared with when the panel was photographed.

true_rho = {"red": 0.06, "nir": 0.40}
light_ratio = {"red": 0.70, "nir": 0.80}      # light at image / light at panel

def ndvi(r): return (r["nir"] - r["red"]) / (r["nir"] + r["red"])

raw = {b: true_rho[b] * light_ratio[b] for b in true_rho}      # no light correction
fixed = {b: raw[b] / light_ratio[b] for b in raw}              # corrected with DLS
for name, r in (("true", true_rho), ("no DLS correction", raw), ("with DLS correction", fixed)):
    print(f"{name:<20} red {r['red']:.3f}  NIR {r['nir']:.3f}  NDVI {ndvi(r):.3f}")
true                 red 0.060  NIR 0.400  NDVI 0.739
no DLS correction    red 0.042  NIR 0.320  NDVI 0.768
with DLS correction  red 0.060  NIR 0.400  NDVI 0.739

Both bands read low, and NDVI reads slightly high. If cloud passes over only half the field, the map shows a boundary between the two halves that has nothing to do with the crop. Flying under uniform sky matters as much as calibration.

Band alignment

A multispectral camera uses a separate lens for each band, each looking from a slightly different position. Without band alignment, the same pixel in each band may be a different spot on the ground, and field bunds show false index values along their edges. MicaSense’s imageprocessing code (MIT licence) includes examples of panel calibration and image alignment that can be tried without a camera.

Module lab

Lab: calibrating multispectral images

  1. Install the imageprocessing code following the project’s guide (Git LFS is required) and open the sample images
  2. Find the panel area in the image, compute the mean DN, and use Example 1 to calibrate the red and NIR bands
  3. Compare NDVI from raw DN with NDVI from reflectance and record the difference
  4. Compare images before and after band alignment and look at misaligned object edges
  5. If the lab has a camera, photograph the panel before and after a real flight, recording time, sky conditions and DLS values in the lab notebook

Common mistakes

Watch out

  • Computing indices from raw DN and comparing across days
  • Using one calibration line for all bands
  • Photographing a shaded or dirty panel, or at an angle that catches glare
  • Flying while clouds pass intermittently with no light data
  • Skipping band alignment

Summary

  • DN depends on light and camera; reflectance is a surface property and must be derived before analysis
  • The CRP provides the reference; the DLS corrects for light changes during flight
  • The empirical line fits a straight line from at least two reference panels, band by band
  • Uneven light and misaligned bands produce false index patterns

Check your understanding

  1. The 5% panel gives DN 3,200 and the 50% panel DN 26,000. What is the gain of the line?
  2. Using question 1, what is the reflectance of a pixel with DN 14,600?
  3. A raw reflectance of 0.042 was captured when light was at 70%. What is it after correction?
  4. Why must calibration be done band by band?
  5. What does skipping band alignment do to an index map?
Answers
  1. DN per unit reflectance
  2. Offset , so
  3. Each band has a different sensitivity and offset
  4. Index values are wrong along object edges, because each band sees a different spot

Key formulas

Empirical line (per band)
Correcting for changing light

Key references

  1. MicaSense. RedEdge-P multispectral sensor [Product specifications]. EagleNXT. link
  2. MicaSense. imageprocessing: MicaSense RedEdge and Altum image processing tutorials (MIT License) [Software]. GitHub. link
  3. Smith, G. M., & Milton, E. J. (1999). The use of the empirical line method to calibrate remotely sensed data to reflectance. International Journal of Remote Sensing, 20(13), 2653–2662. link
  4. Sampath, A., Shrestha, M., While, M., & Scholl, V. M. (2023). Guidelines for calibration of uncrewed aircraft systems imagery (Open-File Report 2023–1033). U.S. Geological Survey. link
  5. Lillesand, T., Kiefer, R. W., & Chipman, J. (2015). Remote sensing and image interpretation (7th ed.). Wiley. link

Further reading

Study the assigned knowledge units in advance, review media and take the module quiz

In class / field

Intensive lab and field practice recorded in a lab notebook

Learning evidence: Lab notebook signed by the instructor

Module quiz

This is a formative self-check, not a graded exam

Knowledge domain: Surveying, mapping and geoinformatics · Sensors and embedded systems